Mechanism of action of Escherichia coli heat stable enterotoxin in a human colonic cell line.
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Mechanism of action of Escherichia coli heat stable enterotoxin in a human colonic cell line.
Escherichia coli heat stable enterotoxin (STa) caused Cl- secretion across T84 cell monolayers in a dose-dependent manner only when applied to the apical membrane surface and not when applied to the basolateral surface. Measurement of cAMP, cGMP, and free cytosolic Ca2+ in response to STa suggested that cGMP alone mediated the Cl- secretory response. Studies utilizing blockers of the Na+,K+-ATP...
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Hepatitis B surface antigen is the first genetically engineered vaccine licensed for human use. Various strategies have been proposed to obtain a vaccine that would bypass the need for injection. In this study, a non-toxic portion of heat-stable enterotoxin of Escherichia coli that is capable of adhering to epithelial cells was inserted at amino acid position 112 of hepatitis surface antigen. T...
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Holmgren, J. (1981) Nature (London) 292, 413417 Hughes, J . M., Murad, F., Chang, B. & Guerrant, R. L. (1978) Nature (London) 271, 755-756 Hughes, S., Warhurst, G., Turnberg, L. A,, Higgs, N. B., Giugliano, L. G. & Drasar, B. S. (1983) Gut 24 (2), 94-98 James, C., Dibley, M., Burke, V., Robinson, J. & Gracey, M. (1982) Clin. Expt. Immunol. 47, 3 4 4 2 Leppla, S. H. (1982) Proc. Natl Acad. Sci. ...
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The properties of a synthetically produced peptide composed of the same primary structure of 18 amino acids described for human Escherichia coli heat-stable enterotoxin were compared with those of purified heat-stable toxin obtained by bacterial growth. The dosage required to evoke fluid secretion in the suckling mouse and rat ligated ileal loop assays was the same for both toxins. The antigeni...
متن کاملProtection in rats immunized with Escherichia coli heat-stable enterotoxin.
Rats immunized with a semipurified preparation of the Escherichia coli heat-stable (ST) enterotoxin conjugated with a protein carrier were protected against challenge with semipurified or purified ST and viable organisms of multiple heterologous serotypes that produce only ST (LT-/ST+), but they were not protected against heal-labile (LT) toxin or viable strains which produce LT either alone (L...
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ژورنال
عنوان ژورنال: Journal of Clinical Investigation
سال: 1988
ISSN: 0021-9738
DOI: 10.1172/jci113626